Salivary Metabolites in Patients with Mucopolysaccharidosis

• Published in: Pesquisa brasileira em odontopediatria e clínica integrada Vol. 24
• Main Authors: Rafaela de Oliveira Torres, Andréa Vaz Braga Pintor, Tatiana Kelly da Silva Fidalgo, Ana Paula Canedo Valente, Liana Bastos Freitas-Fernandes, Ivete Pomarico Ribeiro de Souza
• Format: Journal Article
• Language: English
• Published: Association of Support to Oral Health Research (APESB) 01.11.2023
• Subjects: Magnetic Resonance Spectroscopy; Metabolomics; Mucopolysaccharidoses; Saliva
• ISSN: 1519-0501; 1983-4632

Objective: To identify the salivary metabolites profile of Mucopolysaccharidosis (MPS) types I, II, IV, and VI patients. Material and Methods:The participants were asked to refrain from eating and drinking for one hour before sampling, performed between 7:30 and 9:00 a.m. Samples were centrifuged at 10.000 × g for 60 min at 4ºC, and the supernatants (500µl) were stored at -80ºC until NMR analysis. The salivary proton nuclear magnetic resonance (1H-NMR) spectra were acquired in a 500 MHz spectrometer, and TOCSY experiments were used to confirm and assign metabolites. Data were analyzed descriptively. Results: Differences in salivary metabolites were found among MPS types and the control, such as lactate, propionate, alanine, and N-acetyl sugar. Understanding these metabolite changes may contribute to precision medicine and early detection of mucopolysaccharidosis and its monitoring. Conclusion: The composition of low molecular weight salivary metabolites of mucopolysaccharidosis subjects may present specific features compared to healthy controls.