Full-length infectious clone of a low passage dengue virus serotype 2 from Brazil

Full-length dengue virus (DENV) cDNA clones are an invaluable tool for many studies, including those on the development of attenuated or chimeric vaccines and on host-virus interactions. Furthermore, the importance of low passage DENV infectious clones should be highlighted, as these may harbour cri...

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Bibliographic Details
Published inMemórias do Instituto Oswaldo Cruz
Main Authors Jefferson José da Silva Santos, Tereza Magalhães, José Valter Joaquim Silva Junior, Andréa Nazaré Monteiro Rangel da Silva, Marli Tenório Cordeiro, Laura Helena Vega Gonzales Gil
Format Journal Article
LanguageEnglish
Published Fundação Oswaldo Cruz (FIOCRUZ) 01.01.2015
Subjects
flavivirus
infectious clone
yeast homologous recombination
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Summary:Full-length dengue virus (DENV) cDNA clones are an invaluable tool for many studies, including those on the development of attenuated or chimeric vaccines and on host-virus interactions. Furthermore, the importance of low passage DENV infectious clones should be highlighted, as these may harbour critical and unique strain-specific viral components from field-circulating isolates. The successful construction of a functional Brazilian low passage DENV serotype 2 full-length clone through homologous recombination reported here supports the use of a strategy that has been shown to be highly useful by our group for the development of flavivirus infectious clones and replicons.
ISSN:1678-8060