Mineralization regulation of MAGE-D1 on bone marrowmesenchymal stem cells in knockout mice

Objective To investigate the effect of melanoma associated antigen D1 (Mage-D1) on mouse femoral bone mass and mineralization ability of mouse bone marrow mesenchymal cells (BMSCs) and its potential molecular mechanism. Methods Female Mage-D1 gene knockout heterozygous mice and male wild-type (WT) m...

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Published inLu jun jun yi da xue xue bao Vol. 46; no. 18; pp. 2069 - 2080
Main Authors LU Mingjie, YUAN Hongyan, XU Dan
Format Journal Article
LanguageChinese
Published Editorial Office of Journal of Army Medical University 01.09.2024
Subjects
bone mesenchymal stromal cells
gene knockout
mage-d1
mineralization regulation
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Summary:Objective To investigate the effect of melanoma associated antigen D1 (Mage-D1) on mouse femoral bone mass and mineralization ability of mouse bone marrow mesenchymal cells (BMSCs) and its potential molecular mechanism. Methods Female Mage-D1 gene knockout heterozygous mice and male wild-type (WT) mice were subjected as parent mice to breed Mage-D1 gene knockout homozygous (Mage-D1 KO) mice. PCR and agarose gel electrophoresis were used to identify male Mage-D1 knockout (Mage-D1 KO) mice and littermate male wild-type (WT) mice. Micro-CT scanning was performed to observe mouse femoral bone mass, and ELISA and chemical assay were employed to detect serum levels of calcium, phosphorus, calcitonin, and parathyroid hormone in mice. After primary cultured BMSCs were identified with flow cytometry, immunofluorescence staining was utilized to detect the expression of Mage-D1 in BMSCs. BMSCs were infected by Mage-D1 silencing lentivirus, and then the cells were divided into negative control group (sh-NC) and silencing
ISSN:2097-0927
DOI:10.16016/j.2097-0927.202403078