Analysis of TP53 Codon 72 Gene Polymorphism in Patients with Endometriosis in Isfahan

• Published in: Majallah-i dānishkadah-i pizishkī-i Iṣfahān. (Online) Vol. 29; no. 157; pp. 1509 - 1516
• Main Authors: Mehdi Nikbakht Dastjerdi, Bahram Eslami Farsani, Roshanak Abutorabi, Rasul Salehi, Mohammad Hossein Sanei
• Format: Journal Article
• Language: Persian
• Published: Isfahan University of Medical Sciences 01.12.2011
• Subjects: Endometriosis; Polymorphism; TP53 codon 72 gene
• ISSN: 1027-7595; 1735-854X

Background: Endometriosis is a common disease among women which is a result of endometrial tissue growth outside the uterus. However, its cause is still unclear. This study surveyed TP53 codon 72 gene polymorphism among 90 patients suffering from endometriosis and 90 healthy subjects, as the control group, in Isfahan. Methods: Different genotypes of TP53 codon 72 gene were determined by polymerase chain reaction (PCR). Findings: The frequency of Arg/Arg (Arginine/Arginine) genotype in subjects with endometriosis and healthy individuals were 28.9% and 42.2%, respectively. In addition, the frequency of Pro/Pro (Proline/Proline) genotype in patients with endometriosis and healthy participants were 15.6% and 3.3%, respectively. The frequency of heterozygotes Arg/Pro was 55.6% in endometriosis patients and 54.45% in healthy subjects. Comparing the frequency of Pro/Pro genotype with the two other genotypes in both groups revealed a statistically significant difference between the control and endometriosis groups [P < 0.05; CI = 95%; OR = 5.34 (range: 1047-19.29)].   Conclusion: The present research showed that Pro/Pro genotype of TP53 codon 72 gene is a predisposing genetic factor for endometriosis occurrence in Isfahan.