Preparation of an amino hybrid mesoporous silica-based nanotopography protective coating on a titanium implant surface and evaluation of its osteogenic effect

• Published in: Kou qiang ji bing fang zhi = Journal of prevention and treatment for stomatological diseases Vol. 32; no. 5; pp. 341 - 349
• Main Authors: DU Shimin, LIU Yunxian, CHANG Xiaofeng, LI Zhe
• Format: Journal Article
• Language: Chinese
• Published: Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases 01.05.2024
• Subjects: amination; cell adhesion; mesoporous silicon material; nanocoating; osseointegration; osteoblast; titanium implant; topography induction
• ISSN: 2096-1456
• DOI: 10.12016/j.issn.2096⁃1456.2024.05.003

Objective To deposit degradable amino-hybrid mesoporous silica (AHMS) in situ on the surface of titanium nanotube (TNT) and explore its protective effect on nanomorphology and osteogenesis. Methods TNT and TNT@AHMS were sequentially prepared via an anodizing method: the oil-water two-phase method (experimental group) and the acid-etched titanium method [control group (Ti)]. The parameters for synthesis were explored by changing the silicon source dosage ratio (3∶1, 1∶1, 1∶3); the surface morphology was observed by scanning electron microscope(SEM), hydrophilicity was detected by Water Contact Angle Tester, elemental composition was detected by X-ray photoelectron spectroscopy (XPS); nanoindentation test and ultrasonic oscillator were used to observe the morphological holding effect as mechanical strength of TNT@AHMS in vitro; simulated immersion experiments in vitro was used to observe the degradation behavior of the material. the MC3T3-E1 cell line was used to observe the effect of cell adhesion, proliferati