Study on the efficacy of artemisia oil for acne and the underlying mechanisms in mice

• Published in: Pifu-xingbing zhenliaoxue zazhi Vol. 31; no. 6; pp. 374 - 380
• Main Authors: Hu HUANG, Zhibo YANG, Ziyan QIN, Kan TAO, Huailong CHANG, Lili GUO, Zhanghua HE, Biyao MAO
• Format: Journal Article
• Language: Chinese
• Published: editoiral office of Journal of Diagnosis and Therapy on Dermato-venereology 01.06.2024
• Subjects: acne; artemisia annua oil; inflammation; p. acnes
• ISSN: 1674-8468
• DOI: 10.3969/j.issn.1674-8468.2024.06.002

Objective To assess the effect of artemisia annua oil (AN) on skin damage and inflammation in a mouse model of acne. Methods Forty-eight mice were randomly divided into normal, model, vehicle-treated and different concentrations of AN-treated groups (0.5% AN, 1.0% AN, 1.5% AN, 3.0% AN, 5.0% AN), with 6 mice per group. Except for the normal controls, the other mice were intradermally injected with P. acnes to induce acne. When the acne model was successfully established, mice in model group were fed normally. Mice in vehicle-treated group were sprayed with 1 mL of normal saline, while AN-treated groups were sprayed with 1 mL of different concentrations of AN for 7 days. The skin condition on the back of mice was observed and the skin area of acne was measured. After the treatment, serum and skin tissue were collected. HE staining was used to observe the pathological changes in the skin. ELISA assay was used to measure the serum levels of inflammatory cytokines, including IL-6, IL-1β and TNF-α. Results Five day