Increased expression of G protein-coupled receptor 84 after cerebral ischemia-reperfusion in rats

• Published in: Ji chu yi xue yu lin chuang = Jichu yixue yu linchuang = Basic medical sciences and clinics Vol. 41; no. 3; pp. 398 - 403
• Main Author: ZHAO Chong-chong, GUO Wen-ting, CAI Hong-bin, WANG Huan
• Format: Journal Article
• Language: Chinese
• Published: Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College 01.03.2021
• Subjects: gpr84|neuron|cerebral ischemia|apoptosis
• ISSN: 1001-6325

Objective To determine the expression and function of G protein-coupled receptor 84 (GPR84) in rat cerebral ischemia-reperfusion(I/R). Methods The rat middle cerebral artery occlusion (MCAO) models were performed, and rats were then divided into sham group, cerebral ischemia for 2 h and reperfusion for 12 h group (I/R 12 h), 24 h group (I/R 24 h), 48 h group (I/R 48 h), 72 h group (I/R 72 h); rat primary cortical neurons were isolated and cultured in vitro, neurons were then divided into control group (control), oxygen-glucose deprivation (OGD) for 1 h and recovery for 24 h group (OGD/R 1 h), OGD for 2 h and recovery for 24 h group (OGD/R 2 h); neurons were transfected with GPR84 small interfering RNA (siRNA), cells were divided into control group, OGD/R 1 h group, neurons pretreated with negative control and exposed to OGD for 1 h and recovery for 24 h group (NC+OGD/R 1 h), neurons pretreated with GPR84 siRNA and exposed to OGD for 1 h and recovery for 24 h group (GPR84 siRNA+OGD/R 1 h). Western blot was use