Protective effect of equol on bone in postmenopausal rats with nonalcoholic fatty liver disease

• Published in: Lu jun jun yi da xue xue bao Vol. 45; no. 5; pp. 407 - 416
• Main Authors: CUI Hanqiang, NI Xiangmin, ZHANG Guiming, XU Zhe, LI Shuo, WANG Jian
• Format: Journal Article
• Language: Chinese
• Published: Editorial Office of Journal of Army Medical University 01.03.2023
• Subjects: equol; insulin-like growth factor-1; non alcoholic fatty liver disease; osteoporosis; postmenopause
• ISSN: 2097-0927
• DOI: 10.16016/j.2097-0927.202210126

Objective To observe the osteoprotective effect of equol (Eq) on postmenopausal rats with nonalcoholic fatty liver disease (NAFLD), and to explore its underlying mechanism. Methods Forty-eight 6-week-old female Sprague-Dawley (SD) rats were randomly divided into control group, model group, low-, middle- and high-dose Eq groups [20, 40 and 80 mg/(kg·d)], and estradiol intervention group [E2, 0.25 mg/(kg·d)], with 8 rats in each group. The control group was given sham operation and normal diet. The other groups were given ovariectomy (OVX) combined with high-fat diet. After 16 weeks, the body weight and length of rats were measured, BMI and Lee's index were calculated, and the changes of bone mineral density (BMD) and bone microstructure of rats were detected. After anesthesia, the rats were killed to detect the levels of serum estrogen, TNF-α and IL-6. The liver and femur tissues were observed for morphological changes, and the expression of insulin-like growth factor-1 (IGF-1) in the liver and bone was detect