The effect of bupivacaine on the proliferation and metastasis of tumor cells in an in vitro model of perineural invasion of cholangiocarcinoma and its mechanism

• Published in: 精准医学杂志 Vol. 40; no. 4; pp. 303 - 308
• Main Author: CHEN Menshou, FENG Yujie
• Format: Journal Article
• Language: Chinese
• Published: Editorial Office of Journal of Precision Medicine 01.08.2025
• Subjects: bile duct neoplasms|bupivacaine|neuropilin-1|neurons|axons|neoplasm invasiveness|cell proliferation|cell movement
• ISSN: 2096-529X
• DOI: 10.13362/j.jpmed.202540046

Objective To investigate the effect of bupivacaine on the proliferation and metastasis of tumor cells in an in vitro model of perineural invasion of cholangiocarcinoma and its mechanism. Methods Human cholangiocarcinoma cell line (QBC939 cells) were divided into QBC939 group (group A), QBC939+well-differentiated pheochromocytoma cell line (PC12) group (group B), and QBC939+PC12+bupivacaine group (group C). The cells in group A were cultured in the QBC939 cell-specific medium for 48 h, those in group B were co-cultured with well-differentiated PC12 cells for 48 h in addition to the treatment in group A to establish the in vitro model of perineural invasion of cholangiocarcinoma, and those in group C were cultured with bupivacaine for 48 h in addition to the treatment in group B. CCK-8 assay, EDU staining, cell scratch assay, and Transwell assay were used to measure cell viability and assess the proliferation, migration, and invasion abilities of the three groups of cells. Well-diffe-rentiated PC12 cells were d