Expression of Opa interacting protein 5 in pancreatic cancer and its effect on the proliferation of PANC-1 cells

• Published in: Ji chu yi xue yu lin chuang = Jichu yixue yu linchuang = Basic medical sciences and clinics Vol. 40; no. 6; pp. 803 - 810
• Main Author: ZHOU Hong-ming, TANG Xiao-niu, GUO Wei, LYU Ye-chao, JIANG Yu-xin
• Format: Journal Article
• Language: Chinese
• Published: Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College 01.06.2020
• Subjects: opa interacting protein 5|pancreatic cancer|proliferation|apoptosis
• ISSN: 1001-6325

Objective To explore the expression of OPA interacting protein 5 (OIP5) in pancreatic cancer and its effect on the proliferation of PANC-1 cells. Methods The expression of OIP5 in pancreatic cancer and adjacent tissues was evaluated by database; the expression of OIP5 mRNA and protein in human pancreatic cancer cell lines like MIAPaCa-2, PANC-1, KP-3 and BxPC-3 was detected by real-time quantitative PCR (RT-qPCR) and Western blot, respectively; the expression of OIP5 gene silencing plasmid (pGCSIL-shOIP5)and control plasmid(pGCSIL-shCtrl)were observed, respectively infected with PANC-1 cells, then divided into OIP5 gene silencing group and shctrl control group. 5 days later, RT-qPCR and Western blot were used to detect slow virus knockdown efficiency, and flow cytometry was used to detect cell apoptosis. MTT and cell count were carried out in OIP5 gene silencing group and shctrl control group for 5 consecutive days. OIP5 gene silencing group and shctrl control group were incubated for 10 days to form colonies