Molecular type and antimicrobial resistance analysis of diarrheagenic Escherichia coli in Wuhan from 2019 to 2021

ObjectiveThis study aimed to investigate the distribution of virulence genes， antibiotic resistance， and molecular types of diarrheagenic Escherichia coli （DEC） in Wuhan， Hubei Province， China.MethodsDEC strains identified through biochemical means and a time-of-flight mass spectrometer were routine...

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Published inZhongguo shipin weisheng zazhi Vol. 35; no. 8; pp. 1212 - 1219
Main Authors FEI Xiaoyuan, CHEN Xiaohong, ZHAO Ying, ZHOU Junbo, HUANG Liehong, LONG Yibing
Format Journal Article
LanguageChinese
Published The Editorial Office of Chinese Journal of Food Hygiene 01.08.2023
Subjects
diarrheagenic escherichia coli
minimum spanning tree
multidrug resistance
multilocus sequence typing
pulsed field gel electrophoresis
virulence genes
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Summary:ObjectiveThis study aimed to investigate the distribution of virulence genes， antibiotic resistance， and molecular types of diarrheagenic Escherichia coli （DEC） in Wuhan， Hubei Province， China.MethodsDEC strains identified through biochemical means and a time-of-flight mass spectrometer were routinely collected from five medical institutions. Multiple polymerase chain reactions and drug sensitivity tests were carried out. The molecular characteristics of DEC strains were determined using pulsed field gel electrophoresis （PFGE） and multi-locus sequence typing. Homology analysis was carried out based on clustering analogy and the minimum spanning tree.ResultsA total of 59 DEC strains were collected， including 29 strains of enterotoxigenic Escherichia coli （ETEC， 49.15%）， 15 strains of enteroaggregative Escherichia coli （EAEC， 25.42%）， and 15 strains of enteropathogenic Escherichia coli （EPEC， 25.42%）. The resistance rates of DECs to the 11 antibiotics （except polymyxin E） varied， and were especially high for th
ISSN:1004-8456
DOI:10.13590/j.cjfh.2023.08.012