Preparation of okadaic acid and dinophysistoxins-1 reference material in the mixed matrix of Pectinidae and Prorocentrum lima

• Published in: Zhongguo shipin weisheng zazhi Vol. 34; no. 4; pp. 680 - 686
• Main Authors: WANG Wei, YIN Xinying, CHA Ruimei, XU Dunming, XIAO Jing, CAO Jijuan
• Format: Journal Article
• Language: Chinese
• Published: The Editorial Office of Chinese Journal of Food Hygiene 01.07.2022
• Subjects: dinophysistoxins-1 （dtx1）; mixed matrix reference material; okadaic acid （oa）; pectinidae; preparation; prorocentrum lima
• ISSN: 1004-8456
• DOI: 10.13590/j.cjfh.2022.04.008

ObjectiveTo prepare the standard sample for quantitative analysis of okadaic acid （OA） and dinophysistoxins-1 （DTX1） based on the mixed matrix of Pectinidae and Prorocentrum lima.MethodsPectinidae tissue and algae mud of Prorocentrum lima containing OA and DTX1 were used as raw materials. The matrix OA and DTX1 reference materials were prepared by mixing and freeze-drying. The qualitative confirmation， uniformity and stability test and collaborative constant value analysis were carried out.ResultsIt was confirmed that the reference materials contained OA and DTX1. The homogeneity was good by variance analysis （F test）. The long-term stability and short-term stability under simulated transportation conditions were investigated， and the materials performance was stable. The characteristic standard value and uncertainty of OA and DTX1 in the reference materials were （7.039 ± 0.272） mg/kg （k = 2） and （2.012 ± 0.021） mg/kg （k = 2）， respectively.ConclusionThe mixed matrix reference material can fill the absence of