Optimization of extraction conditions for volatile flavor compounds in crayfish boiled liquid based on HS-SPME-GC-MS

• Published in: Shipin Yu Jixie Vol. 38; no. 2; pp. 57 - 63
• Main Authors: YANG Qi-fu, XU Wen-si, HU Si-si, MA Jia-chen, HUANG Di, LIU Li-ping, HE Jiang, YANG Pin-hong, ZHOU Shun-xiang
• Format: Journal Article
• Language: English
• Published: The Editorial Office of Food and Machinery 01.07.2022
• Subjects: boiled liquid; crayfish; gas chromatography-mass spectrometry (gc-ms); headspace solid phase microextraction (hs-spme); volatile flavor compounds
• ISSN: 1003-5788
• DOI: 10.13652/j.issn.1003-5788.2022.02.010

Objective: In order to increase the added value of crayfish by-products. Methods: With chromatographic peaks and peak areas as the inspection indicators, the optimization on equilibrium time, addition of sodium chloride, extraction time and extraction temperature were optimized, and the composition of volatile flavor compounds was analyzed. Results: The optimal extraction conditions were equilibrium time 0 min, extraction temperature 75 ℃, extraction time 50 min, addition of sodium chloride 30% with 50/30 μm DVB/CAR/PDMS solid phase microextraction head. Under these conditions, a total of 34 volatile flavor compounds, including 5 alcohols, 12 aldehydes, 5 alkanes, 5 aromatic, 5 heterocyclic compounds and 2 esters, were detected in the crayfish boiled liquid. The total peak area was 5.54×108, and the comprehensive score was 95.47. The relative error between the experimental results and the predicted value was 4.53%. Conclusion: The 34 volatile flavor compounds can be extracted from the crayfish boiled liquid by headspace solid phase microextraction.