In vitro establishment of Pinus caribaea var. caribaea apical shoots

• Published in: Biotecnología vegetal Vol. 8; no. 1
• Main Authors: Manuel de Feria, Maité Chávez, Raúl Barbón, Mariana La O, Marta Pérez, Felipe Jiménez-Terry, Elisa Quiala, Daniel Agramonte
• Format: Journal Article
• Language: English
• Published: Universidad Central Marta Abreu de Las Villas 01.01.2008
• ISSN: 1609-1841; 2074-8647

Two shoots types with different morphological characteristics and approximately after 20 days of sprouting from plants cultivated in a donors bank in greenhouse were studied in this research with the objective of establishing in vitro apical shoots of Pinus caribaea var. caribaea. Results demonstrated that the in vitro response of shoots type (B) was superior to shoots type (A) for all the evaluated variables, even when last shoot type was used with 35 days of sprouting. Shoots type (B) presented a 90% of survival, the smallest percentage of damaged shoots (13.3%) and 96.3% of developed shoots. Effect of different concentrations of 6-BAP (0.0; 2.22; 4.44; 6.66 and 8.88 μM) with shoots type (B) in the in vitro establishment phase was evaluated. Results showed that using 6.66 μM 6-BAP the biggest longitudes in shoots (2.58 cm) and the best percentage of them with augmented axillary buds (70%) were achieved. The last characteristic is very important for the later response of the shoots in the multiplication phase. Key words: 6-BAP, growth regulators, pine