ERIC-PCR ANALYSIS OF Riemerella anatipestifer ISOLATES OF KERALA

Molecular characterisation of six Riemerella anatipestifer isolates were carried out by enterobacterial repetitive intergenic consenses polymerase chain reaction (ERICPCR). On analyzing the ERIC-PCR profile, ten to twelve bands per isolate were observed, with a size of approximately 150 bp to 1000 b...

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Published inJournal of Veterinary and Animal Sciences Vol. 48; no. 2; pp. 81 - 85
Main Authors V.S. Sabnam, P.M. Priya, C. Shancy, Radhika Syam, M. Mini
Format Journal Article
LanguageEnglish
Published Director of Academics and Research, Kerala Veterinary and Animal Sciences University 01.07.2017
Subjects
dna finger printing
eric-pcr
kerala
new duck disease
riemerella anatipestifer
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Summary:Molecular characterisation of six Riemerella anatipestifer isolates were carried out by enterobacterial repetitive intergenic consenses polymerase chain reaction (ERICPCR). On analyzing the ERIC-PCR profile, ten to twelve bands per isolate were observed, with a size of approximately 150 bp to 1000 bp. The distance matrices were calculated using the GelQuest - DNA fingerprint analysis software and it was used to construct the phylogenetic relationships among the isolates by the neighbour-joining method. The isolate RA2 was distinct from others. The isolates RA1, RA3, RA4, RA5 having same band pattern approximately at 700 bp were grouped together and separated from RA6. The existence of genetic heterogeneity between the isolates analysed were noticed.
ISSN:0971-0701
2582-0605