Effect of Curcumin on Angiogenesis in Aortic Ring Model of the Wistar Rat

• Published in: Majallah-i dānishgāh-i ̕ulūm-i pizishkī va khadamāt-i bihdāshtī-darmānī Shahīd Ṣadūqī Yazd Vol. 22; no. 3; pp. 1226 - 1236
• Main Authors: J Baharara, M Mousavi, T Ramezani
• Format: Journal Article
• Language: Persian
• Published: Shahid Sadoughi University of Medical Sciences 01.08.2014
• Subjects: Angiogenesis; Aorta; Curcumin; Rat
• ISSN: 2228-5741; 2228-5733

Introduction: Tumeric is a plant with both food and medical properties by which Curcumin is derived from. It has various pharmacological effects. Angiogenesis, a dynamic process of endothelial cells proliferation in order to develop new blood vessels from the previous ones, affects a wide range of physiological and pathological processes such as tumor growth and metastasis. In this study, anti angiogenic effects of Cucumin were investigated in aortic ring of Wistar rats. Methods: In this experimental study, Aortic ring was cut up in to 1 mm pieces and cultured in collagen matrix. After three days, sprouting angiogenesis were observed, and then aortic rings were treated with Curcumin at concentration of 25, 50 and 100 µg/ml. Effects of treatment in all cases were photographed and then investigated by invert microscope. Lengths of vessels were measured by Image J software. Moreover, the study data were analyzed using SPSS in significant level of P<0.05. Result: Data analysis revealed that no significant difference was observed in regard with average of length and number of blood vessels in the control group compared with sham-exposed group (P>0.05).In fact, average length and number of blood vessels in experimental group 1 demonstrated no significant difference compared with control group, though in the experimental group 2 (79.45±3.2mm), (12±1.3) and 3 (38.93±1.1mm), (8±1.1) significant differences were observed (P<0.05(. Conclusion: The results proposed that the Curcumin had dose-dependent inhibitory effects on angiogenesis in rat aortic ring Therefore, it can be introduced as an appropriate candidate in order to study angiogenesis and related diseases.