miR-767-5p inhibits the invasion of ovarian cancer cell lines by regulating the expression of TBL1XR1 protein

• Published in: Ji chu yi xue yu lin chuang = Jichu yixue yu linchuang = Basic medical sciences and clinics Vol. 43; no. 3; pp. 408 - 414
• Main Author: LENG Haina, ZHANG Yan, WANG Xiaodong, ZHANG Xueyou, LI Hongli
• Format: Journal Article
• Language: Chinese
• Published: Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College 01.03.2023
• Subjects: ovarian cancer|invasion|mir-767-5p|transductin beta-like 1 x-linked receptor(tbl1xr1)
• ISSN: 1001-6325
• DOI: 10.16352/j.issn.1001-6325.2023.03.408

Objective To explore the effect and mechanism of miR-767-5P and transductin β1X linked receptor protein 1 (TBL1XR1) on the progression of ovarian cancer. Methods Database analysis: KEGG (Kyoto Encyclopedia of Genes and Genomes, Kyoto Encyclopedia) enrichment analysis of miR-767-5P using GEO, miRPATH database and RStudio TOP5 microRNAs with differential expression multiples were selected from low and high expression microRNAs in the GEO data set, then ranked from low to high in terms of differential multiples. The target genes for miR-767-5p were predicted by miRDB and other databases. The intersection was selected. The target genes were screened by the Open Target Platform, GEPIA and other databases. GEPIA and THPA databases were used to analyze the expression level of TBL1XR1 in human organs, immunohistochemical analysis and survival curve analysis. Experimental verification: Dual luciferase assay detected the binding target of miR-767-5P and TBL1XR1. Western blot was used to detect TBL1XR1 expression in ova