The role of cathepsin K in cardiac dysfunction in mice with alcoholic cardiomyopathy and its damage mechanism

• Published in: Lu jun jun yi da xue xue bao Vol. 44; no. 13; pp. 1330 - 1337
• Main Authors: TANG Yifeng, LIU Zhijiang, XU Guanxue, ZHAO Ranzun, CHEN Wenming, SHI Bei
• Format: Journal Article
• Language: Chinese
• Published: Editorial Office of Journal of Army Medical University 01.07.2022
• Subjects: alcoholic cardiomyopathy; ampk/nox4 signaling; cardiac function; cathepsin k; mice
• ISSN: 2097-0927
• DOI: 10.16016/j.2097-0927.202112025

Objective To investigate the role of cathepsin K (CatK) in cardiac dysfunction in alcoholic cardiomyopathy (ACM) mice and its damage mechanism. Methods Forty-eight C57BL/6J male mice (8 weeks old) were randomly divided into control group (n=12, fed with isocaloric maltodextrin control liquid diet) and model group (n=36, fed with 4% ethanol Lieber-DeCarli liquid diet). After 8 weeks, the mice in the model group identified with decreased cardiac systolic function by echocardiography were regarded as ACM mice, and they were randomly divided into ACM group, sh-NC group, and sh-CatK group, with 12 mice in each group. The mice in the sh-NC group and the sh-CatK group were injected with 1×109 vg (100 μL) of recombinant adenoviruses containing scramble-shRNA or CatK-shRNA via tail vein, respectively. At the end of the 12th week, cardiac function was measured by M-mode echocardiography. The expression of CatK and Nox4 was detected by immunohistochemical assay. In in vitro study, H9c2 cardiomyocytes were used to evalua