The effects of Vitreous Humor on Mesenchymal Stem Cells Derived from Bone Marrow to Lens Fiber Like Cells

• Published in: Armaghān-i dānish Vol. 16; no. 3; pp. 233 - 244
• Main Authors: H Mohseni Kouchesfahani, M Nabiuni, H Delaviz, KH Bahrebar, P Gheibi, N Eslami
• Format: Journal Article
• Language: Persian
• Published: Yasuj University Of Medical Sciences 01.08.2011
• Subjects: Bone Marrow; Fiber; Lens; Stem Cells; Vitreous
• ISSN: 1728-6506; 1728-6514

Introduction & Objective: The function and transparency of the lens actually diminishes with aging as a result, external intervention is necessary to amend it. The aim of the present study was to investigate the effects of vitreous humor on the mesenchymal stem cells (MSCs) to lens fiber like cells. Materials & Methods: The present experimental study was conducted at Tarbiat Moallem University in 2010. Bone marrow was collected from tibias in NMRI mice and then cultured. Immuno-cytochemistery was done to confirm the mesenchymal stem cells using Oct4 antibody. Subsequently, MSCs were treated with bovine vitreous body for 7 and 21 days. The morphology of the MSCs to the lens fibers were studied using Phase Contrast Microscope. Results: During the primary culture, the cell population was heterogeneous and in the subsequent passages, the number of the spindle-shaped cells increased. Immuno-cytochemistery study confirmed the MSCs. Morphological studies showed that most cells in the experimental group were locally longer and more aligned in parallel compared to control group cells. Moreover, lens fiber like with large nuclei and multiple nucleoli was observed. Furthermore, the concentration of 25% of vitreous body had more induction effect on MSCs in comparison with the 50% concentration. Conclusion: The MSCs derived from mouse bone marrow could differentiate into lens fiber like cells by treating them with vitreous humor.