Evaluation of different formulations of a dengue-2 chimeric protein and outer membrane vesicles from Neisseria meningitidis in mice

• Published in: Biotecnología Aplicada Vol. 26; no. 3; pp. 209 - 213
• Main Authors: Iris Valdés, Olivia Niebla, Lisset Hermida, Jorge Sánchez, Laura Lazo, Jorge Martín, Yaremis Romero, Yadira Rodríguez, Maria G Guzmán, Gerardo
• Format: Journal Article
• Language: English
• Published: Elfos Scientiae
• Subjects: adyuvante; neisseria meningitidis; proteína recombinante; vesículas de membrana externa; virus dengue
• ISSN: 1027-2852

New generation vaccines, particularly those based on recombinant proteins, are generally less reactogenic than traditional live attenuated vaccines. Nevertheless, in terms of immunogenicity, they require potent adjuvants to reach a proper immune response in the recipients. We had previously evaluated the potential capacity of PD5 protein (a vaccine candidate against dengue-2, composed by the P64k protein of Neisseria meningitidis, and the domain III of the dengue Envelope protein), as a vaccine candidate with Freund’s adjuvant. In this work, we evaluated the adjuvant capacity of the outer membrane vesicles (OMV) from N. meningitidis on the immunogenicity of the PD5 protein. As a result, after three doses in mice, the groups immunized with three different formulations of OMV elicited high titers of antiviral and neutralizing antibodies against dengue-2 with predominant IgG1 levels. Additionally, in the protection study, the most statistical difference was obtained in one of the three groups immunized with OMV, specifically with one formulation which favors the possible association between the protein and vesicles.