Validation of the XTT-test for assessing the antiproliferative activity of biologics on the basis of monoclonal antibodies

Using the example of Herceptin (Roche) a method of assessing the antiproliferative activity of monoclonal antibodies using tetrazolium salt was developed. The method is based on spectrometric measurement of the level of formazan transformed by living cells from a water soluble salt XTT. The level of...

Full description

Saved in:
Bibliographic Details
Published inBiopreparaty no. 1; pp. 45 - 50
Main Authors I. V. Lyagoskin, M. A. Berestovoy, D. A. Poteryaev, E. S. Zeinalova, A. Yu. Vishnevskiy, A. A. Kazarov
Format Magazine Article
LanguageRussian
Published Ministry of Health of the Russian Federation. Federal State Budgetary Institution «Scientific Centre for Expert Evaluation of Medicinal Products 01.02.2018
Subjects
accuracy
biologics
bt-474
calibration curve
cancer
monoclonal antibodies
precision
specificity
trastuzumab
validation
xtt
биопрепараты
валидация
вт-474
линейность
моноклональные антитела
правильность
прецизионность
рак
специфичность
трастузумаб
хтт
Online AccessGet full text

Cover

More Information
Summary:Using the example of Herceptin (Roche) a method of assessing the antiproliferative activity of monoclonal antibodies using tetrazolium salt was developed. The method is based on spectrometric measurement of the level of formazan transformed by living cells from a water soluble salt XTT. The level of formazan is proportional to the number of viable cells. The technique was in terms of specificity, calibration curve, accuracy and precision.
ISSN:2221-996X
2619-1156