Study of Nigella sativa L. seed’s hydroethanolic extract on skin wound healing in male diabetic rats

• Published in: Armaghān-i dānish Vol. 22; no. 4; pp. 419 - 430
• Main Authors: E Noorbar, N Mirazi
• Format: Journal Article
• Language: Persian
• Published: Yasuj University Of Medical Sciences 01.07.2017
• Subjects: Blackheads; Diabetes mellitus; Rat; Skin ulcer
• ISSN: 1728-6514; 1728-6514

Abstract Background and Aim: Skin wounds are a common complication of diabetes. The use of herbal medicines has had a beneficial effect on healing of the wound. The aim of this study was to evaluate the effect of hydrotanol black currant extract on skin ulcers in diabetic rats. Methods: The current experimental study was performed on 49 male Wistar rats (weighing 250-220 gr) in 7 equal groups. Healthy, untreated, healthy subjects received 1% phenytoin, healthy treated with aspirin (sham), untreated diabetic, diabetic groups (receiving 1% phenytoin, 20% and 40% hydrotagonal extract of blackcurrant). Diabetes was induced by Streptozotocin (60 mg / kg). After anesthesia, injuries were made in the 1 × 2 cm area at the back of each animal, and each group received the treatment for their entire healing until complete healing. Histological studies were performed on tissue samples. The area of ​​the wound was measured every three days once, and the data were analyzed by one-way ANOVA and Tukey's post hoc test. Results: The mean wound healing time for non-treated diabetic groups and phenytoin-treated diabetics was 27 and 24 days, respectively. In healthy groups without treatment, the healthy phine thyinea hemorrhage was completed on days 23, 24 and 21, respectively. The shortest healing time was observed in diabetic groups treated with 20% (18 days) and 40% (15 days) black currant extracts. The two groups also had the lowest mean surface area of ​​the wound during the study period and the mean of the wound levels of the treated groups with the black yeast extract was significantly different from the control group (p<0.05). Conclusion: The extract of black currant has significant effects on the healing process of wounds in rats. Regarding the anti-inflammatory and anti-microbial properties of black cumin, it can be used to repair skin lesions. The antioxidant material in this plant accelerates the process of wound healing in comparison with conventional drugs.