Protective effect of equol on mitochondrial damage induced by high glucose and fat in osteoblasts

• Published in: Lu jun jun yi da xue xue bao Vol. 45; no. 11; pp. 1152 - 1160
• Main Authors: LI Zhengzhou, NI Xiangmin, BAI Qian, LIANG Xinyu
• Format: Journal Article
• Language: Chinese
• Published: Editorial Office of Journal of Army Medical University 01.06.2023
• Subjects: apoptosis; equol; high-glucose and high-fat; mitochondria; oxidative stress; ros1728 cells
• ISSN: 2097-0927
• DOI: 10.16016/j.2097-0927.202210171

Objective To observe the effect of equol (Eq) intervention on osteoblasts induced by high glucose and fat, and to explore its mechanism of action. Methods After ROS1728 osteoblasts were treated with different concentrations of Eq, sodium palmitate (PA) and glucose (GLU) for 48 h, nuclear apoptosis was observed by HE staining and cell survival rate was detected by CCK-8 assay in order to determine the optimal concentrations of GLU, PA and Eq interventions to induce cellular model of diabetic osteoporosis. ROS1728 cells were then divided into control group (5.5 mmol/L GLU), model group (30 mmol/L GLU+250 μmol/L PA), and low, medium and high dose Eq treatment groups (model+1×10-8, 1×10-7 and 1×10-6 mol/L Eq). CCK8 assay was used to detect cell viability, and flow cytometry for cell apoptosis. Hoechst33342 staining and HE staining were employed to observe apoptotic nuclei. Mitochondrial structure, mitochondrial membrane potential (MMP) changes, and protein levels of Sirt1, PGC-1α, Bcl-2, Bax and Cleaved caspase-3