Determinação simultânea de topiramato, carbamazepina, fenitoína e fenobarbital em plasma empregando cromatografia a gás com detector de nitrogênio e fósforo

• Published in: Química Nova Vol. 36; no. 5; pp. 720 - 724
• Main Authors: Roberta Zilles Hahn, Olyr Celestino Kreutz, Marina Venzon Antunes, Rafael Linden, Juliana da Silva, Cléber Álvares da Silva
• Format: Journal Article
• Language: English
• Published: Sociedade Brasileira de Química 01.01.2013
• Subjects: gas chromatography nitrogen phosphorus detector; TMAH; topiramate
• ISSN: 1678-7064
• DOI: 10.1590/S0100-40422013000500018

Topiramate and the other frequently co-administered antiepileptic drugs carbamazepine, phenytoin and phenobarbital were determined in 100 µL plasma samples by gas chromatography with nitrogen phosphorus detection (GC-NPD), after a one-step liquid-liquid extraction with ethyl acetate, followed by flash methylation with trimethylphenylammonium hydroxide. Total chromatographic run time was 12.5 min. Intra-assay and inter-assay precision was 2.5-7.3% and 1.6-5.2%, respectively. Accuracy was 100.1-104.2%. The limit of quantitation was 1 µg mL-1 for all analytes, proving suitable for routine application in therapeutic drug monitoring of antiepileptic drugs.