Application of Specific Fragment Length Polymorphism of Algae rDNA in Identification of Drowning Cases#br

• Published in: Fa yi xue za zhi Vol. 34; no. 5; pp. 516 - 519
• Main Author: YUAN Wen-yong, TANG Xiao-hui, ZHOU Shun-ping，et al
• Format: Journal Article
• Language: Chinese
• Published: Editorial Office of Journal of Forensic Medicine 01.10.2018
• Subjects: forensic pathology; forensic genetics; diatoms; dna, ribosomal; second internal transcribed spacer; amplified fragment length polymorphism analysis; death from drowning
• ISSN: 1004-5619
• DOI: 10.12116/j.issn.1004-5619.2018.05.016

Objective To identify the drop-off location of victims in drowning cases, and confirm whether it is a fatal drowning or the victim is thrown into the water after death by detecting part of 5.8S sequence and second internal transcribed spacer （ITS2） （5.8S+ITS2） of diatom rDNA in water and organs. Methods Two cases identified by diatom examination, which received by Nanjing Municipal Public Security Bureau Forensic Center, were taken as the research objects. The difference of the population structure of algae in water and human tissue was analysed by length polymorphism of 5.8S+ITS2 marker. Results In case 1, similar species of diatom were detected from victim’s lung and liver tissues and the water sample. Two kinds of DNA fragments with length of 330 bp and 376 bp were detected from victim’s lung tissue and the water sample using 5.8S+ITS2 marker, which could confirm the victim was drowning before death. In case 2, there was no diatom found in victim’s lung and liver tissues. Only one kind of DNA fragment with