Function of molecular chaperones in the secretion of two-partner-secretion to the periplasm in Gram-negative bacteria

Objective To investigate the function of general chaperones in the secretion of two-partner-secretion (TPS) protein across the inner membrane to the periplasm of Gram-negative bacteria. Methods The general chaperones were knocked-out and the strains were used to prepare spheroplast to see the effect...

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Published inJi chu yi xue yu lin chuang = Jichu yixue yu linchuang = Basic medical sciences and clinics Vol. 40; no. 4; pp. 456 - 459
Main Author ZHANG Yan-juan, WANG Zhe, CAI Kun, Huang Pi-ying, CHU Yin-di, FAN En-guo
Format Journal Article
LanguageChinese
Published Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College 01.04.2020
Subjects
two-partner-secretion|molecular chaperone|periplasm|gene knock-out|gram-negative bacteria
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Summary:Objective To investigate the function of general chaperones in the secretion of two-partner-secretion (TPS) protein across the inner membrane to the periplasm of Gram-negative bacteria. Methods The general chaperones were knocked-out and the strains were used to prepare spheroplast to see the effects on the secretion of FhaB*, which is a substrate of the representative model TPS substrates FhaB/FhaC system. Results Single knock-out of chaperones Skp,DegP,PpiD,YfgM and double knock-out of PpiD/YfgM has no or little effect on the secretion of FhaB* into the periplasm, while single knock-out of SurA or double knock-out of Skp/DegP severely affect the secretion of FhaB*. Conclusions There may be two molecular chaperone pathways mediated by SurA or by both SKP and DegP in Gram-negative bacteria, which can mediate the substrate protein of the two-partner-secretion system secretes into the peripheral space.
ISSN:1001-6325