Comparison of angiogenetic effects of different dose fractionated radiotherapy on rectal cancer cells

• Published in: Lu jun jun yi da xue xue bao Vol. 44; no. 19; pp. 1979 - 1987
• Main Authors: TONG Chaoxu, ZHANG Tao, GONG Huiying, MAO Weiling, LI Longhao
• Format: Journal Article
• Language: Chinese
• Published: Editorial Office of Journal of Army Medical University 01.10.2022
• Subjects: hif-1α; radiotherapy; rectal cancer; vascular endothelial cells; vegf
• ISSN: 2097-0927
• DOI: 10.16016/j.2097-0927.202206093

Objective To explore the effects of different dose fractionated radiotherapy on angiogenesis in rectal cancer and its potential mechanism. Methods The pathological data of 56 rectal cancer patients admitted to our hospital from 2017 to 2020 were collected for statistical analysis. The microvessel density (MVD) in the rectal cancer tissues under hypofractionated radiotherapy (HFR) and conventional fractionated radiotherapy (CFR) were respectively detected by immunohistochemistry. The expression levels of hypoxia inducible factor 1-alpha (HIF-1α) and vascular endothelial growth factor (VEGF) in the rectal cancer tissues were determined by Western blotting. Then the cultured rectal cancer SW1463 cells and human umbilical vein endothelial cells (HUVECs) were irradiated at doses of 0, 4, 12 and 24 Gy, respectively. CCK-8 assay was subsequently used to test the proliferation of both cells in each group, Western blotting was performed to measure the levels of VEGF and HIF-1α in SW1463 cells, and ELISA assay was adop