Proanthocyanidins inhibit the secretion of Aβ1-42 induced by Aβ25-35 in human neuroblastoma cell line SH-SY5Y

• Published in: Ji chu yi xue yu lin chuang = Jichu yixue yu linchuang = Basic medical sciences and clinics Vol. 41; no. 5; pp. 704 - 708
• Main Author: LI Qi, ZHANG Xiao-qiang, TAO Yi-fan, ZHU Meng-wen, CUI Dan-dan, SUN Teng-teng
• Format: Journal Article
• Language: Chinese
• Published: Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College 01.05.2021
• Subjects: proanthocyanidins|aβ1-42|wnt/β-catenin
• ISSN: 1001-6325

Objective To investigate the effect of proanthocyanidins(PC) induced by amyloid-beta peptide(Aβ25-35) in human neuroblastoma cell line SH-SY5Y and its possible mechanism. Methods SH-SY5Y cells were exposed to Aβ25-35 to establish a cell injury model in vitro. PC and(or) secretory protein Dickkopf-1 (Dkk1), the endogenic inhibitor of Wnt/β-catenin signaling pathway were used for intervention. The cells were divided into control group, Aβ25-35 group, PC(different concentrations)+Aβ25-35 groups, Dkk1 group, Dkk1+ PC+Aβ25-35 group.The cell viability was evaluated by MTT assay. The expressions of β-catenin, GSK-3β, p-GSK-3β were measured by Western blot. The secreted Aβ1-42 was detected by ELISA. Results Compared with the control group, the cell viability, the expression of β-catenin and p-GSK-3β were reduced in Aβ25-35 treated group(P＜0.05), while the secreted Aβ1-42 was increased (P＜0.05). PC improved the cell viability, increased the expression of β-catenin and p-GSK-3β, and reduced the secreted Aβ1-42 in SH-SY