Dynamic changes of femur microarchitecture in ovariectomized mice

Objective To explore the dynamic changes of femur microstructure in ovariectomized osteoporosis mice at different stages. Methods Female C57 mice (8 weeks old) were randomly divided into sham-operation (Sham) and ovariectomy (OVX) groups. Each group was subdivided into 1-week, 1-month and 3-month su...

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Published inDi 3 jun yi da xue xue bao Vol. 43; no. 17; pp. 1627 - 1633
Main Authors QIU Yu, ZHAO Yueyang, LONG Zhimin, WANG Kejian, XU Ling, HE Guiqiong
Format Journal Article
LanguageChinese
Published Editorial Office of Journal of Third Military Medical University 01.09.2021
Subjects
bone microarchitecture
dynamic change
osteoporosis
ovariectomy
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Summary:Objective To explore the dynamic changes of femur microstructure in ovariectomized osteoporosis mice at different stages. Methods Female C57 mice (8 weeks old) were randomly divided into sham-operation (Sham) and ovariectomy (OVX) groups. Each group was subdivided into 1-week, 1-month and 3-month subgroups on the basis of different postoperative time, which representeing early, middle and late menopause, respectively. The femurs were extracted for HE staining, immunohistochemistry and micro-CT analysis, and the changes in the bone microstructure of cancellous bone in the distal femurs, cortical bone in the middle femurs and the levels of osteogenic associated proteins in each group were evaluated. Results Compared with the Sham group, the bone microstructure of the OVX group showed no significant destruction at 1 week after operation, trabecula fracture and bone marrow cavity enlargement from 1 month to 3 months after operation, and significant changes in CT bone parameters BV/TV, Tb.N and Tb.Sp (P < 0.01, P
ISSN:1000-5404
DOI:10.16016/j.1000-5404.202103089