PROTECTIVE EFFECT OF REMIMAZOLAM ON CEREBRAL NERVES IN A RAT MODEL OF CEREBRAL ISCHEMIA/REPERFUSION INJURY AND ITS MECHANISM

• Published in: 精准医学杂志 Vol. 38; no. 4; pp. 345 - 349
• Main Author: DUAN Mei, LIU Jia, ZHANG Jing, SUN Xiaoli, WANG Shilei
• Format: Journal Article
• Language: Chinese
• Published: Editorial Office of Journal of Precision Medicine 01.08.2023
• Subjects: benzodiazepines|reperfusion injury|brain ischemia|nf-kappa b|proto-oncogene proteins c-akt|signal transduction|cytokines|inflammation
• ISSN: 2096-529X
• DOI: 10.13362/jj.pmed.202304013

Objective To investigate the protective effect of remimazolam on cerebral nerves in a rat model of cerebral ischemia/reperfusion injury and its mechanism. Methods A total of 50 male Sprague-Dawley rats were randomly divided into sham-operation group (Sham group), cerebral ischemia/reperfusion group (I/R group), I/R+remimazolam (RE) group, I/R+RE+LY294002 (LY) group, and I/R+LY group. Neurological deficit score was used to evaluate the degree of neurological dysfunction. After arterial blood samples and brain tissue pathological sections were collected from the rats in each group, 2,3,5-trip-henyltetrazolium chloride staining was performed for the brain tissue sections to calculate cerebral infarct volume ratio; ELISA was used to measure the serum levels of interleukin-6 (IL-6), interleukin-1β (IL-1β), and tumor necrosis factor-α (TNF-α); Western blotting was used to measure the relative expression levels of protein kinase B (AKT), phosphorylated AKT (p-AKT), nuclear factor-kappa B p65 (NF-κB p65), and phospho