Equol intervention ameliorates steatosis in HepG2 cells induced by sodium oleate

• Published in: Lu jun jun yi da xue xue bao Vol. 45; no. 3; pp. 202 - 208
• Main Authors: ZHANG Guiming, NI Xiangmin, CUI Hanqiang, XU Zhe, LI Shuo
• Format: Journal Article
• Language: Chinese
• Published: Editorial Office of Journal of Army Medical University 01.02.2023
• Subjects: equol; hepg2 cells; sodium oleate; steatosis
• ISSN: 2097-0927
• DOI: 10.16016/j.2097-0927.202207014

Objective To observe the effect of equol (Eq) intervention on sodium oleate (NaOL)-induced steatosis in HepG2 cells and study its mechanism of action. Methods HepG2 cells were treated with different concentrations of NaOL (0, 0.12, 0.24, 0.36, 0.48, 0.60 and 0.72 mmol/L) for 48 h to induce cell steatosis. Oil red O staining was used to observe the accumulation of intracellular lipid droplets, and triglyceride (TG) content was measured. CCK-8 assay was employed to determine the optimal concentration of NaOL in the inducement of hepatic steatosis and the concentration range for Eq intervention. Then HepG2 cells were randomly divided into 6 groups: control group, NaOL group, NaOL+low-, medium- and high-dose Eq groups (10-7, 10-6, and 10-5 mol/L), NaOL+estradiol intervention group (E2, 10-7 mol/L). After Eq intervention for 48 h, cellular TG content was detected, and Oil red O staining was adopted to observe the accumulation of intracellular lipid droplets, and the expression of SREBP-1c, FAS, PPARα and CPT-1A at