miR-199-3p inhibits diabetes retinopathy in rats by targeting at VegfA

• Published in: Ji chu yi xue yu lin chuang = Jichu yixue yu linchuang = Basic medical sciences and clinics Vol. 43; no. 7; pp. 1084 - 1089
• Main Author: ZHOU Yue, LIN Jingna
• Format: Journal Article
• Language: Chinese
• Published: Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College 01.07.2023
• Subjects: mir-199-3p|vegfa|diabetic retinopathy|retinal ganglion|retinal cells
• ISSN: 1001-6325
• DOI: 10.16352/j.issn.1001-6325.2023.07.1084

Objective To study the role and mechanism of mir-199-3p in the pathogenesis of diabetic retinopathy (DR). Methods The diabetic model of rats was established by injection of streptozotocin. The expression of miR-199-3p and vascular endothelial growth factor A (VEGFA) in the retina of rats was detected by RT-qPCR. The targeting relationship between miR-199-3p and VegfA in retinal cells was studied by dual luciferase assays. Apoptosis of retinal cells was evaluated by TUNEL staining. Protein of VEGFA and transforming growth factor-β(TGF-β), Hepatocyte growth factor (HGF), pigment epithelium derived factor (PEDF) were detected by Western blot. Results The retinal staining showed that the pathological changes such as retinal capillary dilation and vascular curvature in diabetic rats. The results of reverse transcription showed that the expression of miR-199-3p decreased and VEGFA increased in the retina of diabetic rats significantly. The targeting relationship between miR-199-3p and VegfA was confirmed by dual lu