Evaluation of Kirby-Bauer disc diffusion test for antibiotic susceptibility of Neisseria gonorrhoeae

• Published in: Pifu-xingbing zhenliaoxue zazhi Vol. 30; no. 1; pp. 56 - 59
• Main Authors: Shaozhen YE, Dong LIANG, Lianhui LIANG, Changhui CAI
• Format: Journal Article
• Language: Chinese
• Published: editoiral office of Journal of Diagnosis and Therapy on Dermato-venereology 01.02.2023
• Subjects: agar dilution; antimicrobial susceptibility test; disc diffusion method; neisseria gonorrhoeae
• ISSN: 1674-8468
• DOI: 10.3969/j.issn.1674-8468.2023.01.010

Objective To evaluate the accuracy of disc diffusion method for detecting the susceptibility of Neisseria gonorrhoeae to seven surveillance drugs. Methods The agar dilution method and the disc diffusion method were compared in assessing the susceptibility of 60 N. gonorrhoeae strains to ceftriaxone, cefixime, spectinomycin, azithromycin, ciprofloxacin, tetracycline and penicillin. Comparison of results between the two methods included the categorical agreements, minor errors, major errors, and very major errors of drug susceptibility test. Results The categorical agreements of ceftriaxone, cefixime, spectinomycin, azithromycin, ciprofloxacin, tetracycline and penicillin were 95.0%, 76.7%, 100%, 80.0%, 93.3%, 63.3% and 90.0%, respectively. Among the tested antibiotics, minor errors were found for ceftriaxone, cefixime, ciprofloxacin, tetracycline, and penicillin, with rates of 5.0%, 23.3%, 6.7%, 30.0% and 10.0%, respectively. For azithromycin and tetracycline, the very major error rates were 20.0% and 6.7%, re