3D localization of PKHD1L1 in immunogold labeled outer hair cell stereocilia bundles using FIB‐ SEM (postnatal day 4)

A 3D reconstruction of an anti-PKHD1L1 immuno-gold (10 nm) labeled OHC stereocilia bundle from 387 serial FIB-SEM cross-sections, at 15 nm milling step; yellow, gold beads; blue, stereocilia; grey, cell body. For more details see the original study: https://doi.org/10.1038/s41467-019-11712-w. This m...

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Main Authors Indzhykulian, Artur A., Ivanchenko, Maryna V., Cicconet, Marcelo, Hoor Al Jandal, Xudong Wu, Corey, David P.
Format Streaming Video
LanguageEnglish
Published figshare 20.02.2020
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Summary:A 3D reconstruction of an anti-PKHD1L1 immuno-gold (10 nm) labeled OHC stereocilia bundle from 387 serial FIB-SEM cross-sections, at 15 nm milling step; yellow, gold beads; blue, stereocilia; grey, cell body. For more details see the original study: https://doi.org/10.1038/s41467-019-11712-w. This movie is published with permission from the original study.
DOI:10.6084/m9.figshare.11879349