The compound paraquat dichloride hydrate significantly affects the in vitro growth rate of a Chromobacterium violaceum wild type strain

• Published in: Revista ciências médicas e biológicas Vol. 19; no. 1; p. 54
• Main Authors: Gabriel, Jane Eyre, De Souza, Denise Andréa Silva, De Carvalho, Francisco Allan Leandro, Madeira, Humberto Maciel França
• Format: Journal Article
• Language: English
• Published: 17.06.2020
• ISSN: 1677-5090; 2236-5222
• DOI: 10.9771/cmbio.v1i1.25753

The present study aimed to assess the in vitro growth rate of the C. violaceum wild type strain ATCC12472 when exposed to the compound paraquat dichloride hydrate, one of the most herbicides largely employed in agriculture around the world. The bacterium was monitored using spectrophotometry to estimate the growth rate of bacterial inocula incubated in a nutrient broth medium containing the compound paraquat at different concentrations. Moreover, the number of viable bacteria cells in the samples was also estimated based on colony-forming units on the nutrient broth agar. Results showed that the compound paraquat promoted significant decreases in the growth rate of the C. violaceum bacterium in response to incubation time and dose tested. Significantly lower values of bacterial growth were also corroborated by counting the total number of colony-forming units at final concentrations of 100 (<104 CFU) and 1,000 µg mL-1 (<102  CFU) in comparison to control and 0 µg mL-1 (>108  CFU) on the nutrient broth agar. Thus, the findings described herein report the effects of the compound paraquat dichloride hydrate on the in vitro growth rate of a C. violaceum wild type strain.