Evaluation of the DNA Fragmentation of the Normozoosperms at the Pasteur Institute of Cote D’ivoire

• Published in: Journal of advances in biology & biotechnology Vol. 26; no. 10; pp. 12 - 16
• Main Authors: N’Guessan, Marie Florence, Kouadio, Maxime, Beourou, Sylvain, Méité, Souleymane, Ekissi, N’Gbésso Amos, Coulibaly, Founzegue, Djaman, Allico Joseph, Dosso, Mireille
• Format: Journal Article
• Language: English
• Published: 27.11.2023
• ISSN: 2394-1081; 2394-1081
• DOI: 10.9734/jabb/2023/v26i10658

Sperm DNA fragmentation appears to be an important indicator of male fertility and an important parameter for evaluating the positive prognosis of a pregnancy. The objective of our study was to determine the prevalence of DNA fragmentation of normozoosperms received at the Institut Pasteur of Cote d’Ivoire (IPCI). For this we determined the DNA Fragmentation Index (DFI) of spermatozoa as well as its impact in normozoosperms. The spermogram was carried out according to the criteria of the World Health Organization (WHO) and the integrity of the sperm DNA was verified by the acridine orange test. Of the 18 normozoosperms samples selected, an average of 20.28 ± 11.22% for the DNA fragmentation index was obtained. About 27.78% of the samples presented a high level of DFI which reflects a poor pregnancy prognosis. Thus, 27.78% of normozoosperms men have abnormal DNA which does not allow these individuals to be able to conceive. This study showed that DNA fragmentation is an important factor in sperm quality.