Histomorphometric analysis of mast cells in different regions of human intracranial dura mater

• Published in: Revista Headache Medicine (Online) Vol. 10; no. 2; pp. 60 - 62
• Main Authors: Rosas, Emanuela Paz, Paz, Silvania Tavares, Souza Neta, Ana Clara de, Costa, Raisa Ferreira, Silva, Ana Paula Fernandes da, Freitas, Manuela Figueiroa Lyra de, Valença, Marcelo Moraes
• Format: Journal Article
• Language: English
• Published: 30.06.2019
• ISSN: 2763-6178; 2763-6178
• DOI: 10.48208/HeadacheMed.2019.16

Objective: To analyze mast cell histomorphometry in three different regions of the human intracranial dura mater. Method: Three specimens of dura mater were collected after approval by the Ethics Committee (CAAE No. 57692216.5.0000.5208). Each dura mater was obtained from human cadavers between 7 and 24 hours after death. After collection, the samples were fixed, cut into two fragments and longitudinally placed in the following way: external (periosteum) and internal (meningeal) sides. The fragments (1.5 cm2) were taken from three different regions: proximity of the right middle meningeal artery, the proximity of the left middle meningeal artery and superior sagittal sinus. These fragments were submitted to microtomy (10 μm), stained with 0.1% toluidineblue and analyzed by optical microscopy. The histomorphometric parameters adopted were: the distance from the mast cells to the vessels, the number and if the mast cells were degranulated. Five fields from each case were analyzed. For this analysis, the Image J 1.52a 2019 software was used. Results: A higher number of mast cells was observed in the periosteal layer when compared withthe meningeal layer (p=0.026). When the distribution of the mast cells was evaluated, we observed that the cells were localized in the proximity of the middle meningeal artery (p<0.05). Conclusion: In human dura mater, the mast cells are localized in the proximity of dural arteries.