Effects of sample storage using tumor and tissue preservation reagent on genomic data quality

• Published in: The Journal of immunology (1950) Vol. 208; no. 1_Supplement; pp. 116 - 116.17
• Main Authors: Shi, Xiaoshan, Tran, Aimee, Widmann, Stephanie, Tyznik, Aaron J
• Format: Journal Article
• Language: English
• Published: 01.05.2022
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.208.Supp.116.17

Abstract In-depth understanding of the intrinsic properties and complexity of the tumor microenvironment is crucial to develop both diagnostic and therapeutic strategies for cancer treatment. The tumor microenvironment is composed of various cell types, all of which interact and collectively determine cancer progression and response to therapy. Additionally, tumor cells have a strong ability to adapt to environmental stimuli and can evade treatment modalities. As a result of those features, it is essential to understand the dynamic gene expression profile to decipher tumor heterogeneity and develop efficient and safe therapeutic strategies to fight cancer. Challenges are faced by downstream genomic assay design where tumor specimens cannot be processed immediately in the clinical site. In this case, samples need to be preserved properly to maintain good cell quality and transcriptomic profile. In this work, we propose a storage solution named BD® Tumor and Tissue Preservation Reagent (TTPR). We evaluated the RNA quality of sample storage in this solution up to three days. Expression of cellular stress-related genes were assessed over the storage time course. Importantly, samples stored in TTPR proved compatible with single cell capture workflow using BD RhapsodyTM system and the single-cell gene expression is comparable to that of cells from fresh sample. In conclusion, TTPR is a useful and convenient solution for short-term sample storage prior to downstream genomic assays including single-cell sequencing. For Research Use Only. Not for use in diagnostic or therapeutic procedures. BD, the BD Logo and Rhapsody are trademarks of Becton, Dickinson and Company or its affiliates. © 2021 BD. All rights reserved. MSA Approved 1121 Supported by NA