Resurgence of sperm motility: An effect of antioxidant oils in phenytoin induced toxicity of rat testis

• Published in: The professional medical journal Vol. 29; no. 5; pp. 693 - 697
• Main Authors: Khalique-ur-Rehman, Hina Khan, Syed Muhammad Masood Ali, Sayyada Humaira Masood, Jamil Ahmed Siddiqui, Soomro, Dr. Shahid Hussain
• Format: Journal Article
• Language: English
• Published: 30.04.2022
• ISSN: 1024-8919; 2071-7733
• DOI: 10.29309/TPMJ/2022.29.05.6769

Objective: To evaluate the restoration of sperm motility with the antioxidant oils (Virgin coconut oil and Corn oil) therapy in Phenytoin induced testicular toxicity of rats. Study Design: Experimental Study. Setting: Al-Tibri Medical College and Hospital. Period: November 2019 to March 2020. Material & Methods: Forty-eight male albino rats weighing between 150-250gms were taken from the animal house of Al-Tibri medical college and placed in 4 groups, each with 12 rats. Group A was the control group receiving saline, Group B received Phenytoin only, Group C received Phenytoin and Virgin coconut oil, and Group D received Phenytoin and Corn oil. The rats were euthanized on the 4th, 5th, and 6th week of the study. Their epididymis was cut, and the epididymal fluid was placed under the microscope to determine sperm motility. Data was analyzed using SPSS Version 20.0 with one-way ANOVA followed with Post-Hoc Tukey’s test to compare statistical significance. Results: The significant difference was seen in mean % sperm motility when comparing Group B vs. A (≤0.001) and Group B vs. C (≤0.001) during the 4th, 5th, and 6th. Conclusion: Virgin Coconut Oil and Corn Oil both restored sperm motility. A higher percentage was observed in Virgin Coconut Oil received rats, which proved effective in resurging sperm motility.