EXPRESIÓN DE P53 EN CULTIVO DE CÉLULAS MADRE AISLADAS A PARTIR DE QUISTE RADICULAR INFLAMATORIO

• Published in: SCIENTIARVM Vol. 1; no. 1; pp. 33 - 36
• Main Authors: Jácobo Valdivia, Francis Wendell, Bernabé Ortiz, Julio Cesar, Valero Quispe, Javier, Palomino Valverde, Ivo, Muñoz Del Carpio, Toia Agueda Rossangella
• Format: Journal Article
• Language: English
• Published: 04.07.2015
• ISSN: 2411-8826; 2518-2811
• DOI: 10.26696/sci.epg.0118

The inflammatory root cyst is defined as one of the most frequent inflammatory lesions in the oral cavity where it normally involves the apex of the dental roots, giving frequent pathognomonic clinical and radiological characteristics. The objective of this investigation was to determine the expression of p53 in the culture of isolated stem cells from the inflammatory root cyst. In its methodology, the report of a case of a 69-year-old patient was presented, who underwent surgical intervention to extract pieces 11 and 12, from which the sample was obtained for the isolation and culture of the cyst stem cells. periapical inflammatory, obtaining elongated, flat cells with fibroblastic appearance from day 4 and staining with markers for p53 giving a positive response in the evidence of the p53 gene both in the cytoplasm and in the nucleus of these stem cells. Finally, cells can be obtained from the inflammatory root cyst from 4 to 25 days with the use of a modified protocol, in the end, as a contribution to this article, the evidence of the p53 gene is provided both in the nucleus and in the cell cytoplasm. Keywords: Root cyst, p53 gene, stem cells, isolation and culture