CYTOPROTECTIVE EFFECT OF ETHANOL FRACTION OF VERNONIA AMYGDALINA DEL. LEAVES AGAINST THE VERO CELLS

Objectives: The objective of this study is to assess the cytoprotective effect of ethanol fraction (EtF) of Vernonia amygdalina Del. leaves EtF against Vero cells which induced by hydrogen peroxide (H2O2).Methods: Cytoprotective effects of EtF were analyzed by 3-(4,5-dimethylthiazolyl-2)2,5-diphenil...

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Bibliographic Details
Published inAsian journal of pharmaceutical and clinical research Vol. 11; no. 13; p. 146
Main Authors Safriana, Safriana, Rosidah, Rosidah, Hasibuan, Poppy Anjelisa Zaitun, Satria, Denny
Format Journal Article
LanguageEnglish
Published 26.04.2018
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Summary:Objectives: The objective of this study is to assess the cytoprotective effect of ethanol fraction (EtF) of Vernonia amygdalina Del. leaves EtF against Vero cells which induced by hydrogen peroxide (H2O2).Methods: Cytoprotective effects of EtF were analyzed by 3-(4,5-dimethylthiazolyl-2)2,5-dipheniltetrazolium bromide to Vero cells which induced by 0.8 mM H2O2, apoptosis was determined by flow cytometry, and reactive oxygen species (ROS) expression was analyzed by immunocytochemistry.Results: EtF was showed the largest percentage viable cell (78.75±2.51%) at 50 μg/mL. In the analysis of apoptosis by flow cytometry was showed the percentage of viable cell count of 59.56% and EtF was decreased the expression of ROS.Conclusion: EtF has cytoprotective activity toward Vero cells induced by 0.8 mM H2O2.
ISSN:0974-2441
0974-2441
DOI:10.22159/ajpcr.2018.v11s1.26592