Abstract 175: Evaluation of Sepsis-Related Cytokines on Endothelial Cell Permeability

• Published in: Circulation research Vol. 125; no. Suppl_1
• Main Authors: Campbell, Carmen E, Boisvert, William A
• Format: Journal Article
• Language: English
• Published: 02.08.2019
• ISSN: 0009-7330; 1524-4571
• DOI: 10.1161/res.125.suppl_1.175

Abstract only The endothelium plays a critical role in the progression from sepsis to organ failure because endothelium lines all blood vessels and organs in the body to provide a selective barrier. The endothelium is responsible for dynamically regulating the passage of small molecules, fluid, and cells between the bloodstream and tissues. In sepsis, many factors play a role in compromising the endothelium. Dysfunctional endothelium results in development of capillary leak, tissue hypoperfusion, hypoxia, edema, and shock. This study evaluates the individual contributions immunomodulatory cytokines as well as a potential synergistic effect of cytokine combinations on vascular endothelial permeability. Major components of intercellular junctions including tight junction and associated cell adhesion molecules are also assessed microscopically. Specifically, endothelial cell monolayer response to an array of sepsis-related factors including TNF-alpha, IL-1b, IL-6, IL- 8, IL-10, IL-12, IFN-g, and TGF-b by a novel approach using the in-vitro Electric Cell-substrate Impedance Sensing (ECIS) biosensor model is presented.