Abstract 320: Glucose Consumption In Vascular Cell Types In Response To Angiotensin Ii

• Published in: Arteriosclerosis, thrombosis, and vascular biology Vol. 42; no. Suppl_1
• Main Authors: Torimoto, Keiichi, Okuno, Keisuke, Cicalese, Stephanie M, Eguchi, Satoru
• Format: Journal Article
• Language: English
• Published: 01.05.2022
• ISSN: 1079-5642; 1524-4636
• DOI: 10.1161/atvb.42.suppl_1.320

Abstract only Any experimental outcomes are potentially influenced by extracellular glucose availability and its cellular metabolism in cell culture. However, surveillance of vascular-related journals for the past 5 years demonstrated that less than 20% of articles declared the medium glucose concentration. The present studies were designed to seek ideal medium glucose concentration(s) in vascular cell types with particular attention paid to changes in glucose consumption upon angiotensin II (AngII) stimulation. We have compared glucose consumption in three vascular cell types, endothelial cells (EC), vascular smooth muscle cells (VSMC) and adventitial fibroblasts (AF) with or without 100 nM AngII stimulation. In all cell types after a 48-hour incubation in relatively low glucose media (1 g/L in 6 well dish with 1mL), medium glucose concentration was reduced to almost 0. Whereas medium glucose concentration remained significantly higher (EC 2.77±0.05 g/L, VSMC 3.87±0.05 g/L, AF 3.32±0.01 g/L) when cells were incubated for 48 hours in high glucose (4.5 g/L) media. In middle glucose (2.75 g/L) media, medium glucose concentration remained in physiological ranges (EC 0.62±0.18 g/L, VSMC 1.98±0.07 g/L, AF 1.17±0.17 g/L). AngII treatment enhanced glucose consumption in AF and VSMC but not in EC. Enhanced extracellular acidification rate by AngII was observed in AF. PDGF-BB also stimulated glucose consumption in AF which was associated with a trend of cell proliferation. In AF, AngII induction of target proteins at 48 hours varied depending on the glucose concentration used. In low glucose media induction of GRP78 or hexokinase II was highest, whereas induction of VCAM-1 was lowest. Utilization of specific inhibitors further suggest essential roles of AT1 receptor and glycolysis in AngII-induced fibroblast activation. Overall, the present study demonstrates a high risk of hypo- or hyperglycemic conditions when standard low or high glucose media is used with vascular cells. Moreover, these conditions may significantly alter experimental outcomes. Medium glucose concentration should be monitored during any culture experiments and utilization of middle glucose media is recommended for all vascular cell types.