Metabotropic Regulation of RhoA/Rho-Associated Kinase by L-type Ca 2+ Channels New Mechanism for Depolarization-Evoked Mammalian Arterial Contraction

• Published in: Circulation research Vol. 108; no. 11; pp. 1348 - 1357
• Main Authors: Fernández-Tenorio, Miguel, Porras-González, Cristina, Castellano, Antonio, del Valle-Rodríguez, Alberto, López-Barneo, José, Ureña, Juan
• Format: Journal Article
• Language: English
• Published: 27.05.2011
• ISSN: 0009-7330; 1524-4571
• DOI: 10.1161/CIRCRESAHA.111.240127

Background: Sustained vascular smooth muscle contraction is mediated by extracellular Ca 2+ influx through L-type voltage-gated Ca 2+ channels (VGCC) and RhoA/Rho-associated kinase (ROCK)-dependent Ca 2+ sensitization of the contractile machinery. VGCC activation can also trigger an ion-independent metabotropic pathway that involves G-protein/phospholipase C activation, inositol 1,4,5-trisphosphate synthesis, and Ca 2+ release from the sarcoplasmic reticulum (calcium channel-induced Ca 2+ release). We have studied the functional role of calcium channel-induced Ca 2+ release and the inter-relations between Ca 2+ channel and RhoA/ROCK activation. Methods and Results: We have used normal and genetically modified animals to study single myocyte electrophysiology and fluorimetry as well as cytosolic Ca 2+ and diameter in intact arteries. These analyses were complemented with measurement of tension and RhoA activity in normal and reversibly permeabilized arterial rings. We have found that, unexpectedly, L-type Ca 2+ channel activation and subsequent metabotropic Ca 2+ release from sarcoplasmic reticulum participate in depolarization-evoked RhoA/ROCK activity and sustained arterial contraction. We show that these phenomena do not depend on the change in the membrane potential itself, or the mere release of Ca 2+ from the sarcoplasmic reticulum, but they require the simultaneous activation of VGCC and the downstream metabotropic pathway with concomitant Ca 2+ release. During protracted depolarizations, refilling of the stores by a residual extracellular Ca 2+ influx through VGCC helps maintaining RhoA activity and sustained arterial contraction. Conclusions: These findings reveal that calcium channel-induced Ca 2+ release has a major role in tonic vascular smooth muscle contractility because it links membrane depolarization and Ca 2+ channel activation with metabotropic Ca 2+ release and sensitization (RhoA/ROCK stimulation).