Morphological alterations in minor salivary glands of HTLV 1+ patients: A pilot study

• Published in: Journal of oral pathology & medicine Vol. 47; no. 10; pp. 985 - 990
• Main Authors: do Vale, Daniela Assis, Andrade, Natália Silva, Casseb, Jorge, de Oliveira, Augusto Penalva, Bussolotti Filho, Ivo, Trierveiler, Marilia, Ortega, Karem López
• Format: Journal Article
• Language: English
• Published: 01.11.2018
• ISSN: 0904-2512; 1600-0714
• DOI: 10.1111/jop.12777

Background Among the complex of HTLV ‐associated diseases, Sjögren's syndrome ( SS ) is one of the most controversial. This work aims to detect morphological and inflammatory alterations, including clues of the presence of HTLV ‐1, in minor salivary glands of patients with dryness symptoms. Methods We have assessed HTLV ‐1‐seropositive patients ( HTLV ‐1 group) and patients with SS ( SS group). We used formalin‐fixed, paraffin‐embedded minor salivary gland tissue to evaluate the morphological aspects and, by means of immunohistochemistry, the presence of Tax protein, CD 4, CD 8 and CD 20 cells. Additionally, viral particles and proviral load were analysed by PCR . Results The HTLV ‐1 group had the highest prevalence of non‐specific chronic sialadenitis (85.71%; P  =   0.017) and greater amount of T CD 8 + cells. In the SS group, focal lymphocytic sialadenitis (80%; P  =   0.017) prevailed, with a greater amount of B CD 20 + . Both immunohistochemistry and PCR identified the Tax protein and its gene in the salivary glands of both groups and in similar proportions. Conclusion The results indicate that HTLV ‐1‐seropositive patients have different patterns of morphological/inflammatory alterations, suggesting a likely difference in the process of immune activation.