Bromobimane crosslinking studies in oligomycin‐sensitive ATPase from beef heart mitochondria M r 31 000 protein crosslinked

Using a bromobimane fluorescent label the M r 31 000 protein band oligomycin‐sensitive (OS)‐ATPase from beef heart mitochondria is shown to become much intensified by 2‐mercaptopropionylglycine. In the presence of 3.5 nmol/mg protein of the thiol reagent ATP—P i exchange activity is increased by 90%...

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Bibliographic Details
Published inFEBS letters Vol. 150; no. 1; pp. 207 - 210
Main Authors Zimmer, Guido, Mainka, Luise, Heil, Berthold M.
Format Journal Article
LanguageEnglish
Published 13.12.1982
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Summary:Using a bromobimane fluorescent label the M r 31 000 protein band oligomycin‐sensitive (OS)‐ATPase from beef heart mitochondria is shown to become much intensified by 2‐mercaptopropionylglycine. In the presence of 3.5 nmol/mg protein of the thiol reagent ATP—P i exchange activity is increased by 90%. With the fluorescent crosslinking reagent dibromobimane (DB) we show that a new fluorescent peak appears between M r 50 000 and 60 000. ATP—P i exchange is very much decreased by DB. The results suggest that for regulation of ATP‐synthetase activity sulfhydryl groups in the region of the M r 31 000 protein(s) play an important role.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(82)81335-5