Purification of triterpenoid saponins and 25 R /25 S ‐inokosterone from Achyranthes bidentata Bl. by high‐speed countercurrent chromatography coupled silver nitrate coordination

• Published in: Journal of separation science Vol. 47; no. 7
• Main Authors: Niu, Tong, Yu, Jinqian, Wang, Zhenqiang, Wang, Chuangchuang, Guo, Yingjian, Li, Jian, Wang, Xiao
• Format: Journal Article
• Language: English
• Published: 01.04.2024
• ISSN: 1615-9306; 1615-9314
• DOI: 10.1002/jssc.202300901

An effective method by high‐speed countercurrent chromatography coordinated with silver nitrate for the preparative separation of sterones and triterpenoid saponins from Achyranthes bidentata Blume was developed. Methyl tert‐butyl ether/n‐butanol/acetonitrile/water (4:2:3:8, v/v/v/v) was selected for 20‐hydroxyecdysone (compound 1 ), chikusetsusaponin IVa methyl ester (compound 4 ), 2′‐glycan‐11‐keto‐pigmented saponin V (compound 5 ), as well as a pair of isomers of 25 S ‐inokosterone (compound 2 ) and 25 R ‐inokosterone (compound 3 ), which were further purified by silver nitrate coordinated high‐speed countercurrent chromatography. What is more, dichloromethane/methanol/isopropanol/water (6:6:1:4, v/v/v/v) was applied for calenduloside E (compound 6 ), 3 β ‐[(O‐ β ‐ d ‐glucuronopyranosyl)‐oxy]‐oleana‐11,13‐dien‐28‐oic acid (compound 7 ), zingibroside R1 (compound 8 ) and chikusetsusaponin IVa (compound 9 ). Adding Ag + to the solvent system resulted in unique selectivity for 25 R /25 S isomers of inokosterone, which increased the complexing capability and stability of Ag + coordinated 25 S ‐inokosterone, as well as the α value between them. These results were further confirmed by the computational calculation of geometry optimization and frontier molecular orbitals assay. Comprehensive mass spectrometry and nuclear magnetic resonance analysis demonstrated the structures of the obtained compounds.