Constant Enzymatic in situ Production of H 2 O 2 for an Unspecific Peroxygenase by an l– Amino Acid Oxidase

Unspecific Peroxygenases have been the subject of a lot of research in the recent past as they offer opportunities for green hydroxylation reactions using hydrogen peroxide (H 2 O 2 ) as donor. A striking problem of UPOs is their low stability against their own co‐substrate H 2 O 2 . In order to pre...

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Published inAdvanced synthesis & catalysis Vol. 367; no. 11
Main Authors Last, Simon, Heinks, Tobias, Dietz, Niklas, Koopmeiners, Simon, Fischer von Mollard, Gabriele, Weissenborn, Martin J., von Langermann, Jan
Format Journal Article
LanguageEnglish
Published 03.06.2025
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Summary:Unspecific Peroxygenases have been the subject of a lot of research in the recent past as they offer opportunities for green hydroxylation reactions using hydrogen peroxide (H 2 O 2 ) as donor. A striking problem of UPOs is their low stability against their own co‐substrate H 2 O 2 . In order to prevent the deactivation, we developed an enzyme cascade with an l ‐amino acid oxidase (LAAO) to produce hydrogen peroxide in situ at a constant low concentration, aiming for much higher lifespan and therefore longer working time of the enzyme. This way, we are able to convert a variety of substrates with conversion rates up to 95%.
ISSN:1615-4150
1615-4169
DOI:10.1002/adsc.202500105