PPARG基因Q448H突变位点对中国荷斯坦牛繁殖性状的影响

奶牛（Bos taurus）的繁殖性能与机体能量代谢密切相关,过氧化物酶体增殖物激活受体γ（peroxisome proliferator-activated receptor γ,PPARγ）是由PPARG基因编码的配体激活的核转录因子,在调控动物能量稳态、繁殖生理和免疫应答等过程中发挥关键性作用,PPARG是奶牛繁殖性状的重要候选基因。本实验采用聚合酶链式反应-单链构象多态（polymerase chain reaction products-single strand conformation polymorphism,PCR-SSCP）技术并结合测序,检测中国荷斯坦牛PPARG基因ex...

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Published in农业生物技术学报 Vol. 26; no. 2; pp. 253 - 262
Main Author 贺鹏迦;马正才;敏妍滢;董艳娇;朱静;马永生;马彦男
Format Journal Article
LanguageChinese
Published 2018
Subjects
中国荷斯坦牛
单核苷酸多态性
繁殖性状
过氧化物酶体增殖物激活受体γ基因（PPARγ）
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Summary:奶牛（Bos taurus）的繁殖性能与机体能量代谢密切相关,过氧化物酶体增殖物激活受体γ（peroxisome proliferator-activated receptor γ,PPARγ）是由PPARG基因编码的配体激活的核转录因子,在调控动物能量稳态、繁殖生理和免疫应答等过程中发挥关键性作用,PPARG是奶牛繁殖性状的重要候选基因。本实验采用聚合酶链式反应-单链构象多态（polymerase chain reaction products-single strand conformation polymorphism,PCR-SSCP）技术并结合测序,检测中国荷斯坦牛PPARG基因exon7区Q448H错义突变,并分析其对中国荷斯坦牛繁殖性状的影响。结果表明,PPARG基因exon7共存在2个等位基因3种基因型,等位基因G/T在低代杂种、高代杂种和纯种荷斯坦牛群体中的频率分别为0.65/0.35、0.75/0.25和0.69/0.31,优势基因型为GG型;3个实验群体在该位点均处于中度多态。测序结果显示,与普通牛PPARG基因序列（NC_007320.6）相比,T等位基因在64 947位核苷酸处发生了G→T的碱基突变,导致第448位氨基酸由谷氨酰胺变为组氨酸,而G等位基因与NC_007320.6序列相同。最小二乘法分析表明,GG型产后第一次配种天数（days to first breeding,DFB）显著低于GT型（P〈0.05）。对于G等位基因,DFB、DO和NSC（第2胎次）的加性效应分别为-6.97 d、-11.2 d和-0.215次。基因替代效应分别为-8.83 d、-15.70 d和-0.25次,即每个G等位基因替代T等位基因会导致产后第一次配种天数提前8.83 d,空怀天数缩短15.7 d,配妊次数降低0.25次,等位基因G为高繁殖性能优势基因。PPARG基因Q448H突变位点可作为中国荷斯坦牛繁殖性状的分子标记。本研究为中国荷斯坦牛繁殖性状的标记辅助选择提供了理论依据。
Bibliography:Reproductive performance in dairy cows is highly correlated with energy metabolism. The peroxisome proliferator-activated receptor gamma（PPARγ） is a ligand-activated nuclear transcription factorthat plays an important role in regulating the processes of energy homeostasis, reproductive, and immune physiology. A potentially important candidate gene for reproductive traits of dairy cows is PPARγ, which is encoded by the PPARG. In the present study, a Q448 H mutation of the PPARG gene exon7 in Chinese Holstein cattle was detected with polymerase chain reaction products-single strand conformational polymorphism（PCR-SSCP） and DNA sequencing, and assessed through reproductive traits. Results demonstrate the presence of two alleles and 3 genotypes in the locus of the PPARG gene exon7. The 2 alleles were named G and T. The allelic frequencies of the G/T alleles in the three groups were 0.65/0.35, 0.75/0.25,and 0.69/0.31 for the Low-Hybrids, Improved-Hybrids, and Pure Holstein populations, respectively; the GG genotyp
ISSN:1674-7968