ARR12 promotes de novo shoot regeneration in Arabidopsis thaliana via activation of WUSCHE Lexpression

Auxin and cytokinin direct cell proliferation anddifferentiation during the in vitro culture of plant cells, but themolecular basis of these processes, especially de novo shootregeneration, has not been fully elucidated. Here, we describethe regulatory control of shoot regeneration in Arabidopsistha...

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Published in植物学报：英文版 Vol. 59; no. 10; pp. 747 - 758
Main Author Xuehuan Dai;Zhenhua Liu;Meng Qiao;Juan Li;Shuo Li;Fengning Xiang
Format Journal Article
LanguageEnglish
Published 2017
Subjects
ARR12
Lexpression
promotes
WUSCHE
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Summary:Auxin and cytokinin direct cell proliferation anddifferentiation during the in vitro culture of plant cells, but themolecular basis of these processes, especially de novo shootregeneration, has not been fully elucidated. Here, we describethe regulatory control of shoot regeneration in Arabidopsisthaliana （L.） Heynh, based on the interaction of ARABIDOPSISRESPONSE REGULATOR12 （ARR12） and WUSCHEL （WUS）. Themajor site of ARR12 expression coincided with the locationwhere the shoot apical meristem （SAM） initiated. The arr12mutants showed severely impaired shoot regeneration andreduced responsiveness to cytokinin; consistent with this, theoverexpression of ARR12 enhanced shoot regeneration.Certain shoot meristem specification genes, notably WUSCHEL（WUS） and CLAVATA3, were significantly downregulated in thearr12 explants. Chrornatin immunoprecipitation （CHIP） andtransient activation assays demonstrated that ARR12 binds tothe promoter of WUS. These observations indicate that duringshoot regeneration, in vitro, ARR12 functions as a molecularlink between cytokinin signaling and the expression of shootmeristem specification genes.
Bibliography:11-5067/Q
ISSN:1672-9072
1744-7909